Ayhan Gücüyener received undergraduate education in Galatasaray University Faculty of Economics and Administrative Sciences, Department of International Relations between 2006 and 2012 in French. She finalized her graduate education in Bilgi University Department of International Political Economy with the thesis on "Political Economy of Turkish Azerbaijani Energy Relations". Gücüyener has worked in a Turkish think-tank Center on Foreign Policy and Security as a security researcher from September 2013 to July 2016. She was also coordinating institute's Critical Infrastructure Protection Program. (CIP)

She has attended a numerous international organizations as a lecturer or speaker. She was also co-director and lecturer in the "CIP against Terrorist Attacks" training course organized by COE-DAT (NATO Center of Excellence Defense Against Terrorism).

She is active in IAEA (International Atomic Energy Agency)'s cyber/computer security working groups. In addition, she is a member and previous lecturer in the Global Cyber Security (CS) Alumni Community of Interest (COI) by George C. Marshall European Center for Security Studies in Germany. In March 2017, among 40 professionals in worldwide, she was selected as a Young Professional to attend NATO Transformation Seminar organized in Budapest.

Since February 2017, she has been working as Scientific Associate in Energy Pact which was established in 2007 in Switzerland as a non-profit and non-political organisation, and is now in the process of relocating to Vienna, Austria. Its mission is to promote the balanced use of energy sources with the objective of reconciling innovation, economic development and the protection of the environment.